Jelsen Darrock is a one-man death squad with a merciless streak a mile wide. He was once a part of the Order of Azyr, a well-known and fearsome hunter of witches, aethergheists, and cultists of the Dark Gods, though his conduct saw him expelled. The exact reason remains a guarded secret.
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Increased AR expression and apoptosis upon induction of ES cell differentiation. (a) Caspase-3 activity assay (top panel) and WB analysis (bottom panel) showed a correlation between increasing expression of AR protein and induction of caspase-3 activity in undifferentiated ESCs, differentiated ESCs and EBs. Oct3/4 was employed as a self-renewal marker and β-tubulin as the loading control. (b) DHT-treated ESCs were cultured with LIF or left untreated for the indicated times. Both caspase-3 activity and AR expression showed a time-dependent increase from undifferentiated to differentiated ESCs. (c) Clones stably expressing either shAR or control shRNA (shLuc) were established in ESCs and EBs. (d) Caspase-3 activity assay and WB analysis using caspase-3, -12 and PARP antibodies showed a significant increase in activation of caspase-3 and -12 in control ESCs treated with DHT and T, but not ESCs expressing shAR. N suppressed the androgen-mediated effect in ESCs. (e) TUNEL staining of ESCs and EBs expressing shAR or shLuc. EBs contained more apoptotic cells than ESCs, and knockdown of AR led to significant suppression of cell death in both ESCs and EBs (n=3, *P 2ff7e9595c
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